Transport of S -Adenosylmethionine in Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Regulation of S-adenosylmethionine levels in Saccharomyces cerevisiae.
Methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, used to methylate homocysteine in methionine biosynthesis. Methionine can be activated by ATP to give rise to the universal methyl donor, S-adenosylmethionine (AdoMet). Previously, a chimeric MTHFR (Chimera-1) comprised of the yeast Met13p N-terminal catalytic domai...
متن کاملMutation of Saccharomyces cerevisiae preventing uptake of S-adenosylmethionine.
A mutant has been isolated whose aberration severely restricts the ability of cells of Saccharomyces cerevisiae to take up S-adenosylmethionine. The mutation apparently also affects adenosylhomocysteine uptake, but not that of the S-adenosylmethionine moieties adenine, homocysteine, homoserine, or methionine, nor the sulfonium compound, S-methylmethionine. It is a single, chromosomal mutation w...
متن کاملActive transport of exogenous S-adenosylmethionine and related compounds into cells and vacuoles of Saccharomyces cerevisiae.
Saccharomyces cerevisiae 4094-B (alpha, ade-2, ura-1) in potassium phosphate buffer with glucose under aerobic conditions took up (-)S-adenosyl-l-methionine from the medium in sufficient quantity to permit the demonstration of its accumulation in the vacuole by ultraviolet micrography. The same result was obtained with (+/-)S-adenosyl-l-methionine, (+/-)S-adenosyl-d-methionine, and (-)S-adenosy...
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We used sequence motifs conserved in S-adenosylmethionine-dependent methyltransferases to identify 26 putative methyltransferases from the complete genome of the yeast Saccharomyces cerevisiae. Seven sequences with the best matches to the methyltransferase consensus motifs were selected for further study. We prepared yeast disruption mutants of each of the genes encoding these sequences, and we...
متن کاملSAMl , the Structural Gene for One of the S - Adenosylmethionine Synthetases in Saccharomyces cerevisiae
Saccharomyces cerevisiae contains two genes, SAMl and SAM2, encoding functional S-adenosylmethionine synthetases. The gene SAMl was isolated by functional complementation of a double mutant of S. cerevisiae, and its identity was confirmed by gene disruption. The cloned gene was used to probe wild type chromosomal DNA, and two regions hybridizing with SAMl were found, one of which is the SAMl ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1972
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.109.2.499-504.1972